Journal: Circulation Research

Article Title: Successive Action of Meprin A and Neprilysin Catabolizes B-Type Natriuretic Peptide

doi: 10.1161/circresaha.107.153585

Figure Lengend Snippet: Figure 2. Screening for peptidase(s) degrading BNP. A, Degra- dation of mBNP by kidney membrane of NEP-knockout mice in the presence of effective concentrations of peptidase inhibitors; values are shown as meansSEM (n5). B, Degradation of na- triuretic peptides by recombinant meprin (n3; SD within the

Article Snippet: Recombinant mouse NEP, meprin and subunits, and the cGMP kit were from R&D Systems (Wiesbaden, Germany).

Techniques: Membrane, Knock-Out, Recombinant

Journal: Circulation Research

Article Title: Successive Action of Meprin A and Neprilysin Catabolizes B-Type Natriuretic Peptide

doi: 10.1161/circresaha.107.153585

Figure Lengend Snippet: Figure 4. NEP participation in mBNP7-32 degradation. A, Deg- radation of natriuretic peptides by recombinant NEP (n3; SD within the points); degradation of mBNP (B) and simultaneous formation of mBNP7-32 (C) by wild-type mouse kidney mem- brane in the presence of no inhibitor (white; N), candoxatrilat (horizontally striped; C), actinonin (vertically striped; A), or candoxatrilat and actinonin (crosshatched; AC); control (black, CON) is mBNP without degradation; values are shown as meansSEM (n3). ns indicates not significant. #P0.005 vs no inhibitor.

Article Snippet: Recombinant mouse NEP, meprin and subunits, and the cGMP kit were from R&D Systems (Wiesbaden, Germany).

Techniques: Recombinant, Control

Journal: Journal of Neuropathology & Experimental Neurology

Article Title: Neprilysin and Insulin-Degrading Enzyme Levels Are Increased in Alzheimer Disease in Relation to Disease Severity

doi: 10.1097/nen.0b013e3181afe475

Figure Lengend Snippet: FIGURE 7. Ex vivo modeling of postmortem stability of neprilysin (NEP) and insulin-degrading enzyme (IDE) activities. (AYD) Samples of midfrontal cortex from 2 control brains with postmortem delays of less than 10 hours after storage at 4-C or room temperature (RT) for 6 to 72 hours. No appreciable loss of enzyme activity was observed for NEP (A, B) or IDE (C, D) except in a set of aliquots stored at RT (C, red lines). (E, F) There was no appreciable loss of enzyme activity on incubation of recombinant human NEP (500 ng/mL) and IDE (500 ng/mL) for 0 to 120 minutes at either 4-C or RT. (G, H) Mouse brain tissue homogenates stored between 0 and 320 minutes at 4-C or RT showed no appreciable loss of NEP or IDE enzyme activity. Solid lines indicate best-fit linear regression; interrupted lines indicate 95% confidence intervals. There was no correlation observed between enzyme activity and incubation time at either 4-C or RT.

Article Snippet: After washing, crude brain tissue homogenate or subcellular fractions (250 Kg total protein), diluted in PBS (pH 7.4), were incubated together with serial dilutions of recombinant human NEP (R&D Systems Europe) at RT for 2 hours with continuous shaking.

Techniques: Ex Vivo, Control, Activity Assay, Incubation, Recombinant